Epidermal Growth Factor
Production of bioactive peptides: Epidermal Growth Factor (EGF)
Zera®-EGF has been produced in tobacco plants (stable and transient transformation), mammalian cells (CHO) and insect cells (Sf9 and H5).
1) Zera®-EGF is accumulated inside StorPro® organelles in all tested hosts.
2) Tobacco StorPro® organelles containing EGF have been isolated and solubilized. EGF is released by enzymatic cleavage and subsequently purified by reverse phase chromatography.
3) Cell based proliferation activity assay shows that fusion protein Zera®-EGF has at least 50% of the activity of a commercial EGF. As expected, Zera® domain has no proliferation activity. This result indicates that cleavage is not needed to abtain active EGF.
Additional information and scientific data for the use of Zera® for the production of peptides are available under confidentiality.